Microscope Diffusing Filter Comparison

A diffuser has an interesting texture, as you may expect. I think the attempt is to make a "normal distribution" of cell sizes and random spatial distribution, to make as uniformly random as possible the diffusing action.

These are all shown first for BD Plan in brightfield, then darkfield, and finally with polarizers. When available, M Plan brightfield, polarized and DIC is then presented.

10x

BD diffusing filter 10x BD brightfield BD diffusing filter 10x BD darkfield BD diffusing filter 10x BD polarized M Plan diffusing filter 10x M Plan brightfield M Plan diffusing filter 10x M Plan polarized M Plan diffusing filter 10x M Plan DIC

20x

M Plan diffusing filter 20x M Plan brightfield M Plan diffusing filter 20x M Plan polarized M Plan diffusing filter 20x M Plan DIC

40x

BD diffusing filter 40x BD brightfield BD diffusing filter 40x BD darkfield BD diffusing filter 40x BD polarized

60x

BD diffusing filter 60x BD brightfield BD diffusing filter 60x BD darkfield BD diffusing filter 60x BD polarized M Plan diffusing filter 60x M Plan brightfield M Plan diffusing filter 60x M Plan polarized

100x

BD diffusing filter 100x BD brightfield BD diffusing filter 100x BD darkfield BD diffusing filter 100x BD polarized
M Plan diffusing filter 100x M Plan DIC M Plan diffusing filter 100x M Plan DIC

M Plan diffusing filter 100x M Plan polarized M Plan diffusing filter 100x M Plan polarized

The first two images of the final row are two 100x images with M Plan objectives, and show the effect of restricting the field stop to limit stray light. The second two are different polarizer angles with DIC.


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